RT-PCR / qPCR

Highly sensitive nucleic acid detection and quantification solutions for gene expression analysis, pathogen detection, molecular diagnostics, and research applications.

Expert Scientists

Highly-qualified Scientific team.

Accurate Results

High-quality & reliable data

Online Reporting

Confidential Online Reports

Fast Service

Quick yet Precise Results

Overview

Real-Time PCR (qPCR) and Reverse Transcription PCR (RT-PCR) are powerful molecular techniques used for the detection, quantification, and analysis of DNA and RNA targets. These methods provide highly accurate, rapid, and reproducible results across research, clinical, veterinary, agricultural, and industrial applications.

At Genewise, we utilize advanced real-time PCR platforms and validated workflows to deliver precise quantitative data for gene expression studies, pathogen detection, genotyping, and molecular diagnostics.

About RT-PCR / qPCR

What is RT-PCR / qPCR?

RT-PCR (Reverse Transcription PCR) converts RNA into complementary DNA (cDNA) before amplification, enabling accurate analysis of gene expression and RNA-based targets.

qPCR (Quantitative PCR) monitors DNA amplification in real time using fluorescent detection systems, allowing precise measurement of nucleic acid quantities.

Common Sample Types

RNA Samples

Total RNA, mRNA, and purified RNA extracts.

Genomic DNA

High-quality DNA for quantitative analysis.

Blood Samples

Whole blood, plasma, and serum-derived nucleic acids.

Tissue Samples

Fresh, frozen, or preserved tissues.

Microbial Samples

Bacterial, viral, fungal, and environmental samples.

Cell Cultures

Cultured cells for expression profiling and validation.

Applications

Gene Expression Analysis

Measure mRNA expression levels across biological samples.

Pathogen Detection

Detect bacterial, viral, fungal, and parasitic targets.

Molecular Diagnostics

Support clinical and veterinary diagnostic testing.

Genetic Validation

Confirm gene knockdown, knockout, or transgene expression.

Biomarker Studies

Quantify disease-associated biomarkers.

Agricultural Testing

Analyze plant pathogens and gene expression responses.

DELIVERABLES

Sample Requirements

RNA Samples

Tissue Samples

DNA Samples

General Requirements

Delivery Time

Standard:

7–14 Working Days

Express:

3–5 Working Days (On Request)

Our Testing Process

01

Sample Reciept

We receive and register your sample with unique identification.

02

DNA Extraction

High-quality DNA is extracted using optimized protocols.

03

PCR Amplification

Target gene (COI) is amplified using specific primers.

04

Sequencing

The amplified product is sequenced using advanced sequencing platforms.

05

Data Analysis

Sequencing are compared with global database for accurate identification.

06

Report Delivery

You receive a detailed report with species identification and data.

Consultation & Sample Booking

Trusted by Researchers, Laboratories & Healthcare Professionals

Results Provided
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Trusted across Pakistan for precision diagnostics, advanced sequencing, and molecular testing services. Connect with our experts to discuss your project, request a quote, or receive guidance tailored to your research and diagnostic needs.

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